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Nile tilapia piscidin 2, TP2
General Information
DRACP ID DRACP02332
Peptide Name Nile tilapia piscidin 2, TP2
Sequence GECIWDAIFHGAKHFLHRLVNP
Sequence Length 22
UniProt ID L0CKQ8
PubChem CID Not available
Origin Oreochromis niloticus
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | Low active up to 100 µg/ml | MTS assay | 24 h | 1 |
| RAW 264.7 | Mouse leukemia | Leukemia | Low active up to 100 µg/ml | MTS assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=50 µg/ml; Tilapia erythrocytes: 60% Hemolysis=40 µg/ml
Normal (non-cancerous) Cytotoxicity TO2: ~10% Killing=80 µg/ml; MRC-5: Low active up to 100 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C119H174N34O28S
Absent amino acids MQSTY
Common amino acids H
Mass 293574
Pl 7.62
Basic residues 5
Acidic residues 2
Hydrophobic residues 10
Net charge 3
Boman Index -1783
Hydrophobicity 3.18
Aliphatic Index 93.18
Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 261.9
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23226256
Title Five different piscidins from Nile tilapia, Oreochromis niloticus: analysis of their expressions and biological functions
Doi 10.1371/journal.pone.0050263
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4197
This work is licensed under a Creative Commons Attribution 4.0 International License.