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Nile tilapia piscidin 4, TP4
General Information
DRACP ID DRACP02334
Peptide Name Nile tilapia piscidin 4, TP4
Sequence FIHHIIGGLFSAGKAIHRLIRRRRR
Sequence Length 25
UniProt ID L0CKG3
PubChem CID Not available
Origin Oreochromis niloticus
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | ~80% Killing=22 µg/ml | MTS assay | 24 h | 1 |
| RAW 264.7 | Mouse leukemia | Leukemia | ~60% Killing=22 µg/ml | MTS assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: 100% Hemolysis=12.5 µg/ml; Tilapia erythrocytes: 100% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity TO2: 50% Cell death=20 µg/ml; MRC-5: ~50% Cell death=18 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antiviral
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C135H226N50O27
Absent amino acids CDEMNPQTVWY
Common amino acids R
Mass 340987
Pl 13.2
Basic residues 10
Acidic residues 0
Hydrophobic residues 11
Net charge 10
Boman Index -6561
Hydrophobicity -12.8
Aliphatic Index 117.2
Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23226256
Title Five different piscidins from Nile tilapia, Oreochromis niloticus: analysis of their expressions and biological functions
Doi 10.1371/journal.pone.0050263
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4199
This work is licensed under a Creative Commons Attribution 4.0 International License.