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Temporin-1CEa
General Information
DRACP ID DRACP02396
Peptide Name Temporin-1CEa
Sequence FVDLKKIANIINSIFGK
Sequence Length 17
UniProt ID B8QP36
PubChem CID Not available
Origin Rana chensinensis
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | IC50=31.48 µM | MTT assay | 24 h | 1 |
| BCaP-37 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=37.52 µM | MTT assay | 24 h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Carcinoma | IC50=65.42 µM | MTT assay | 24 h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=50.32 µM | MTT assay | 24 h | 2 |
| BEL-7402 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=38.98 µM | MTT assay | 24 h | 2 |
| LK-2 | Lung squamous cell carcinoma | Carcinoma | IC50=63.4 µM | MTT assay | 24 h | 2 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=53.31 µM | MTT assay | 24 h | 2 |
| NCI-H446 | Lung small cell carcinoma; Small cell lung cancer | Carcinoma | IC50=67.66 µM | MTT assay | 24 h | 2 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=61.63 µM | MTT assay | 24 h | 2 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=36.31 µM | MTT assay | 24 h | 2 |
| HO-8910 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=64.75 µM | MTT assay | 24 h | 2 |
| HT-29 | Colon adenocarcinoma | Carcinoma | IC50>100 µM | MTT assay | 24 h | 2 |
Hemolytic Activity Human erythrocytes: LC50=95.74 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C91H150N22O23
Absent amino acids CEHMPQRTWY
Common amino acids I
Mass 220591
Pl 10.51
Basic residues 3
Acidic residues 1
Hydrophobic residues 9
Net charge 2
Boman Index -470
Hydrophobicity 58.82
Aliphatic Index 137.65
Half Life
Mammalian: 30 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23609760
Title Design of potent, non-toxic anticancer peptides based on the structure of the antimicrobial peptide, temporin-1CEa
Year 2013
Literature 2
Pubmed ID 21871946
Title Antitumor effects and cell selectivity of temporin-1CEa, an antimicrobial peptide from the skin secretions of the Chinese brown frog (Rana chensinensis)
Doi 10.1016/j.biochi.2011.08.011
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4544
This work is licensed under a Creative Commons Attribution 4.0 International License.