This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-1Sa, SEQ ID NO:1 for Patent EP 2853538 A1
General Information
DRACP ID DRACP02399
Peptide Name Temporin-1Sa, SEQ ID NO:1 for Patent EP 2853538 A1
Sequence FLSGIVGMLGKLE
Sequence Length 13
UniProt ID B3KYH4
PubChem CID Not available
Origin Pelophylax saharicus (Sahara frog) (Rana saharica)
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| THP-1 | Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia | Leukemia | IC50>60 μM | MTT assay | 72 h | Patent |
| HepG2 | Hepatoblastoma | Blastoma | LC50=560 μM | MTT assay | 72 h | Patent |
Hemolytic Activity Rat erythrocytes: LC50=25 µM
Normal (non-cancerous) Cytotoxicity Human fibroblasts: LC50>100 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C63H106N14O17S
Absent amino acids ACDHNPQRTWY
Common amino acids GL
Mass 157791
Pl 6.41
Basic residues 1
Acidic residues 1
Hydrophobic residues 6
Net charge 0
Boman Index 1611
Hydrophobicity 118.46
Aliphatic Index 142.31
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID Not available
Title Not available
Doi Not available
Year Not available
Patent
Patent ID EP 2853538 A1
Patent Title Analogues of temporin-SHa and uses thereof
Other Iinformation Patent Application; Family: 11s / 11ex; Family Jurisdictions: CA, EP, JP, BR, US, WO, ES; Legal Status: Discontinued; Application No: 13306344; Filed: Sep 27, 2013; Published: Apr 1, 2015; Earliest Priority: Sep 27, 2013
Other Published ID BR112016006424A2 CA2924035A1 EP3049432A1 EP3049432B1 ES2691323T3 JP2016539658A JP6669656B2 US2016280749A1 US9932376B2 WO2015044356A1
This work is licensed under a Creative Commons Attribution 4.0 International License.