This work is licensed under a Creative Commons Attribution 4.0 International License.
Kalata B1 [N15Y]
General Information
DRACP ID DRACP02585
Peptide Name Kalata B1 [N15Y]
Sequence GLPVCGETCVGGTCYTPGCTCSWPVCTRN
Sequence Length 29
UniProt ID P56254
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Active ACP Membrane lysis
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=8.1±0.4 μM | Resazurin assay | 2, 24 h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=12.3±0.6 μM | Resazurin assay | 2, 24 h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=3.2±0.1 μM | Resazurin assay | 2, 24 h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=6.9±0.3 µM
Normal (non-cancerous) Cytotoxicity HFF-1: CC50=6.7±0.6 µM; PBMCs: CC50=1.0±0.1 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C122H190N34O40S6
Absent amino acids ADFHIKMQ
Common amino acids C
Mass 346503
Pl 5.94
Basic residues 1
Acidic residues 1
Hydrophobic residues 5
Net charge 0
Boman Index -1301
Hydrophobicity 22.76
Aliphatic Index 43.45
Half Life
Mammalian: 2.8 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 7365
Absorbance 280nm 263.04
Polar residues 19
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25099014
Title Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_12200
This work is licensed under a Creative Commons Attribution 4.0 International License.